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Tuning Net Charge in Aliphatic Polycarbonates Alters Solubility and Protein Complexation Behavior

Published

Author(s)

Nicholas D. Posey, Julia Danischewski, Michael Lueckheide, Yuanchi Ma, Jeffrey Fagan, Vivek Prabhu

Abstract

A synthetic strategy yielded polyelectrolytes and polyampholytes with tunable net charge for complexation and protein binding. Organocatalytic ring-opening polymerizations yielded aliphatic polycarbonates that were functionalized with both carboxylate and ammonium side chains in a post-polymerization, radical-mediated thiol−ene reaction. Incorporating net charge into the polymer architecture altered the chain dimensions in phosphate buffered solution in a manner consistent with selfcomplexation and complexation behavior with model proteins. A net cationic polyampholyte with 5% of carboxylate side chains formed large clusters rather than small complexes with bovine serum albumin, while 50% carboxylate polyampholyte was insoluble. Overall, the aliphatic polycarbonates with varying net charge exhibited different macrophase solution behaviors when mixed with protein, where self-complexation appears to compete with protein binding and larger-scale complexation.
Citation
ACS Ω
Volume
6

Keywords

aliphatic polycarbonates, polyampholytes, small angle neutron scattering, dynamic light scattering, polymer/protein complexes, self-coacervation

Citation

Posey, N. , Danischewski, J. , Lueckheide, M. , Ma, Y. , Fagan, J. and Prabhu, V. (2021), Tuning Net Charge in Aliphatic Polycarbonates Alters Solubility and Protein Complexation Behavior, ACS Ω, [online], https://doi.org/10.1021/acsomega.1c02523 (Accessed August 12, 2022)
Created August 26, 2021, Updated March 2, 2022