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Tranmission Electron Microscopy with a Liquid Flow Cell

Published

Author(s)

Kate L. Klein, Ian M. Anderson, N. de Jonge

Abstract

The imaging of microscopic structures at nanometer-scale spatial resolution in a liquid environment is of interest for a wide range of studies. Transmission electron microscopy (TEM) provides a method for imaging with the requisite spatial resolution, but such imaging can be accomplished only with a means of isolating the liquid environment from the high vacuum of the instrument column, such as an impermeable cell with electron transparent windows. Recently, a liquid flow cell TEM holder equipped with a miniaturized viewing chamber has been developed and applied to the detection of high atomic number labeling nanoparticles by scanning TEM high-angle annular dark-field imaging. Here we demonstrate the application of the flow cell system for conventional TEM bright-field imaging of immobilized nanoparticles in a liquid of micrometer thickness.
Citation
Journal of Microscopy
Volume
242

Keywords

in situ, liquid, nanoparticles, STEM, TEM

Citation

Klein, K. , Anderson, I. and de, N. (2011), Tranmission Electron Microscopy with a Liquid Flow Cell, Journal of Microscopy, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=906875 (Accessed July 25, 2024)

Issues

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Created January 20, 2011, Updated February 19, 2017