Cicerone, M.
, Dunkers, J.
and Washburn, N.
(2004),
Towards In-Situ Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques, Tissue Engineering, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852112
(Accessed October 6, 2024)
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Towards In-Situ Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques
Published
Author(s)
, , N Washburn
Abstract
Inability to obtain cellular-level information about progress of cell growth in tissue-engineering (TE) scaffolds is a pervasive problem. We demonstrate that a confocal microscope with two collinear contrast mechanisms, optical coherence, and fluorescence, can be used to perform non-destructive imaging of a polymer TE scaffold containing osteoblasts. We show that the combination of the techniques shows promise for in situ measurements of cell growth in a bioreactor, even for highly opaque TE scaffolds.Citation
Tissue Engineering
Pub Type
Journals
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Keywords
coherence, florescence, microscopy, morphology, OCT, scaffold, tissue engineering
Citation
Issues
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Created January 12, 2004, Updated February 19, 2017