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Towards In-Situ Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques

Published

Author(s)

Marcus T. Cicerone, Joy P. Dunkers, N Washburn

Abstract

Inability to obtain cellular-level information about progress of cell growth in tissue-engineering (TE) scaffolds is a pervasive problem. We demonstrate that a confocal microscope with two collinear contrast mechanisms, optical coherence, and fluorescence, can be used to perform non-destructive imaging of a polymer TE scaffold containing osteoblasts. We show that the combination of the techniques shows promise for in situ measurements of cell growth in a bioreactor, even for highly opaque TE scaffolds.
Citation
Tissue Engineering

Keywords

coherence, florescence, microscopy, morphology, OCT, scaffold, tissue engineering

Citation

Cicerone, M. , Dunkers, J. and Washburn, N. (2004), Towards In-Situ Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques, Tissue Engineering, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852112 (Accessed June 18, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created January 12, 2004, Updated February 19, 2017