Towards In-Situ Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques
Marcus T. Cicerone, Joy P. Dunkers, N Washburn
Inability to obtain cellular-level information about progress of cell growth in tissue-engineering (TE) scaffolds is a pervasive problem. We demonstrate that a confocal microscope with two collinear contrast mechanisms, optical coherence, and fluorescence, can be used to perform non-destructive imaging of a polymer TE scaffold containing osteoblasts. We show that the combination of the techniques shows promise for in situ measurements of cell growth in a bioreactor, even for highly opaque TE scaffolds.
, Dunkers, J.
and Washburn, N.
Towards In-Situ Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques, Tissue Engineering, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852112
(Accessed November 30, 2023)