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Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release



Mikhail Zhernenkov, Rana N. Ashkar, Hao Feng, Olukemi O. Akintewe, Nathan D. Gallant, Ryan Toomey, John F. Ankner, Roger Pynn


Thermoresponsive poly(N-isopropylacrylamide) (NIPAAm) has been widely used as a surface coating to thermally control the detachment of adsorbed cells without the need for extreme stimuli such as enzyme treatment. In this context, cell adhesion to 2D and 3D scaffolds, controlling cell positioning, growth, spreading and migration have been of a great interest in tissue engineering and cell biology. Here, we use NIPAAm polymer surface coating to uncontrollably change the surface topography of 2D linear structures using temperature stimuli. Neutron reflectometry and surface diffraction are implemented to the grating, and the evolution of the system in response to temperature variation. Using this proof-of-concept experiment, we show that such a 2D scaffold exhibits surface-tunable properties that can be potentially implemented for controlled tissue growth procedures with a built-in non-extreme tissue-release mechanism.
ACS Applied Materials and Interfaces


polymer coating, cell-growth scaffolds, neutron reflectometry, NIPAAm


Zhernenkov, M. , Ashkar, R. , Feng, H. , Akintewe, O. , Gallant, N. , Toomey, R. , Ankner, J. and Pynn, R. (2015), Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release, ACS Applied Materials and Interfaces, [online], (Accessed June 14, 2024)


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Created June 9, 2015, Updated October 12, 2021