Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release

Published

June 10, 2015

Author(s)

Mikhail Zhernenkov, Rana N. Ashkar, Hao Feng, Olukemi O. Akintewe, Nathan D. Gallant, Ryan Toomey, John F. Ankner, Roger Pynn

Abstract

Thermoresponsive poly(N-isopropylacrylamide) (NIPAAm) has been widely used as a surface coating to thermally control the detachment of adsorbed cells without the need for extreme stimuli such as enzyme treatment. In this context, cell adhesion to 2D and 3D scaffolds, controlling cell positioning, growth, spreading and migration have been of a great interest in tissue engineering and cell biology. Here, we use NIPAAm polymer surface coating to uncontrollably change the surface topography of 2D linear structures using temperature stimuli. Neutron reflectometry and surface diffraction are implemented to the grating, and the evolution of the system in response to temperature variation. Using this proof-of-concept experiment, we show that such a 2D scaffold exhibits surface-tunable properties that can be potentially implemented for controlled tissue growth procedures with a built-in non-extreme tissue-release mechanism.

Citation

ACS Applied Materials and Interfaces

Volume

7

Issue

22

Pub Type

Journals

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Keywords

polymer coating, cell-growth scaffolds, neutron reflectometry, NIPAAm

Citation

Zhernenkov, M. , Ashkar, R. , Feng, H. , Akintewe, O. , Gallant, N. , Toomey, R. , Ankner, J. and Pynn, R. (2015), Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release, ACS Applied Materials and Interfaces, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=917911 (Accessed March 18, 2025)