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A Thermodynamic Study of the Conversion of Chorismate to Isochorismate
Published
Author(s)
Yadu D. Tewari, A M. Davis, Prasad T. Reddy, Robert N. Goldberg
Abstract
Microcalorimetry and high performance liquid chromatography were used to conduct a thermodynamic investigation of the biochemical reaction chorismate(aq) = isochorismate(aq). this reaction occurs at the branch point of the chorismate metabolic pathway that leads to the synthesis of enterobaction. Isochorismate synthase, the enzyme that catalyzes this reaction, was prepared for this study by using molecular biology techniques. The equilibrium and calorimeteric measurements were performed at the temperature T = 298.15 K and at the respective pHs 7.34 and 6.93. For the chemical reference reaction chorismate2-(aq) = isochorismate2-(aq), the equilibrium constant K = (0.84 0.04) and the standard molar enthalpy of reaction δrH m = (0.81 ).27) kJ mol-1 at T = 298.15 K and ionic strength I = 0. Under approximately physiological conditions, the apparent equilibrium constant K' = 0.83 and the standard transformed Gibbs energy change δfG'm = 0.49 kJ mol-1 for the overall biochemical reaction.
Tewari, Y.
, Davis, A.
, Reddy, P.
and Goldberg, R.
(2000),
A Thermodynamic Study of the Conversion of Chorismate to Isochorismate, Journal of Chemical Thermodynamics
(Accessed September 26, 2023)