Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

A standardized approach for the dispersion of titanium dioxide nanoparticles in biological media

Published

Author(s)

Julian S. Taurozzi, Vincent A. Hackley, Mark R. Wiesner

Abstract

We describe a comprehensive optimization study culminating in a standardized and validated approach for the preparation of titanium dioxide (TiO2) nanoparticle dispersions in relevant biological media (PBS and DMEM-FBS). This study utilizes a candidate TiO2 reference nanomaterial based on a commercially relevant powder that has been widely applied in both acute and chronic toxicity studies. The dispersion approach as presented here satisfies four key harmonization requirements not previously addressed: 1) method transferability, based in part on the use of a sonication energy calibration method that allows for power measurement and reporting in a device-independent manner; 2) optimization of sonication parameters and thorough method validation in terms of particle size distribution, pH, isoelectric point, concentration range and powder batch variability; 3) minimization of sonolysis side effects by elimination of organics during sonication, and 4) characterization of nanoparticle agglomeration under various dispersion conditions by use of laser diffraction spectrometry, an in situ size characterization technique that provides advantages over techniques more commonly employed within the context of nanotoxicology (e.g., dynamic light scattering). The described procedure yields monomodal, nanoscale, protein-stabilized nanoparticle dispersions in biological media that remain stable for at least 48 h (acute testing timeframe) under relevant incubation conditions.
Citation
Nanotoxicology
Volume
7
Issue
4

Keywords

nanomaterial, agglomerate, aggregate, bovine serum albumin, toxicology, suspension, colloidal stability

Citation

Taurozzi, J. , Hackley, V. and Wiesner, M. (2013), A standardized approach for the dispersion of titanium dioxide nanoparticles in biological media, Nanotoxicology, [online], https://doi.org/10.3109/17435390.2012.665506 (Accessed February 25, 2024)
Created June 1, 2013, Updated November 10, 2018