Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Spatially patterned matrix elasticity directs stem cell fate

Published

Author(s)

Chun Yang, Frank W. DelRio, Lena Basta, Hao Ma, Kyle Kyburz, Anouk Killaars, Kristi Anseth

Abstract

There is a growing appreciation for the functional role of matrix mechanics in regulating stem cell self-renewal and differentiation. However, it is largely unknown how sub-cellular, spatial mechanical variations in the local extracellular environment mediate intracellular signal transduction and cell fate decisions. Here, the effect of spatial organization and the magnitude of sub-cellular matrix mechanical properties on human mesenchymal stem cells (hMSCs) was investigated using hydrogels fabricated with spatially distinct regions. Highly spread morphologies and higher Yes-associated protein (YAP) activation were observed in hMSCs seeded on hydrogels with higher concentrations of stiff regions. However, randomizing the spatial organization of the stiff regions resulted in rounded morphologies and lower levels of YAP activation. Additionally, disorganization of the matrix mechanics led to differences in cell fate. Collectively, this material platform has allowed innovative experiments to elucidate a novel spatial mechanical dosing mechanism that correlates to the magnitude and organization of spatial stiffness.
Citation
Proceedings of the National Academy of Sciences of the United States of America
Volume
113
Issue
31

Keywords

stem cells, hydrogels, atomic force microscopy

Citation

Yang, C. , DelRio, F. , Basta, L. , Ma, H. , Kyburz, K. , Killaars, A. and Anseth, K. (2016), Spatially patterned matrix elasticity directs stem cell fate, Proceedings of the National Academy of Sciences of the United States of America, [online], https://doi.org/10.1073/pnas.1609731113, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=918632 (Accessed October 21, 2021)
Created July 18, 2016, Updated October 12, 2021