Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

A Simple Approach to One-Laser, Broadband CARS Microscopy



Marcus T. Cicerone, T W. Kee


Coherent anti-Stokes Raman scattering (CARS) microscopy is emerging as a powerful method for imaging materials and biological systems, partly because of its non-invasiveness and selective chemical sensitivity. However, its full potential for species-selective imaging is limited by restricted spectral bandwidth. Recent increases in bandwidth are promising, but still are not sufficient for the level of robust component discrimination as would be needed in a chemically complex milieu found, for example, in intra- and extracellular environments. Here we demonstrate a truly broadband CARS imaging instrument which we use to acquire hyperspectral images with vibrational spectra over a bandwidth of 2500 cm 1, and resolution of 20 cm^- 1.
Optics Letters


CARS, multivariate analysis, raman


Cicerone, M. and Kee, T. (2004), A Simple Approach to One-Laser, Broadband CARS Microscopy, Optics Letters, [online], (Accessed February 29, 2024)
Created December 1, 2004, Updated February 19, 2017