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A Simple Approach to One-Laser, Broadband CARS Microscopy

Published

Author(s)

Marcus T. Cicerone, T W. Kee

Abstract

Coherent anti-Stokes Raman scattering (CARS) microscopy is emerging as a powerful method for imaging materials and biological systems, partly because of its non-invasiveness and selective chemical sensitivity. However, its full potential for species-selective imaging is limited by restricted spectral bandwidth. Recent increases in bandwidth are promising, but still are not sufficient for the level of robust component discrimination as would be needed in a chemically complex milieu found, for example, in intra- and extracellular environments. Here we demonstrate a truly broadband CARS imaging instrument which we use to acquire hyperspectral images with vibrational spectra over a bandwidth of 2500 cm 1, and resolution of 20 cm^- 1.
Citation
Optics Letters
Volume
29
Issue
23

Keywords

CARS, multivariate analysis, raman

Citation

Cicerone, M. and Kee, T. (2004), A Simple Approach to One-Laser, Broadband CARS Microscopy, Optics Letters, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852331 (Accessed February 29, 2024)
Created December 1, 2004, Updated February 19, 2017