Significant Parameters in the Optimization of MALDI-TOF-MS

Published

February 1, 2004

Author(s)

S Wetzel, Kathleen M. Flynn, James J. Filliben

Abstract

One of the most significant issues in any analytical technique is optimization. Optimization and calibration are key factors in quantitation. In matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), one of the limitations restricting quantitation is instrument optimization. Understanding which parameters are most influential and the effects of these parameters on the mass spectrum is required for optimization. This understanding is especially important when characterizing synthetic polymers by MALDI-TOF-MS, due to the complex nature of synthetic polymers. Several instrument parameters were studied using an orthogonal experimental design to understand effects on the signal-to-noise of a polystyrene distribution. The parameters examined included; laser energy, detector voltage, is/2 voltage, lens voltage and delay time. Other factors considered were polymer concentration and matrix. Detector voltage and delay time were the most influential of the instrument parameters for polystyrene.

Proceedings Title

Sigma Xi Postdoctoral Poster Presentations, 2004

Conference Dates

February 19-20, 2004

Conference Location

Undefined

Pub Type

Conferences

Keywords

MALDI-TOF-MS, optimization, parameters, significant

Citation

Wetzel, S. , Flynn, K. and Filliben, J. (2004), Significant Parameters in the Optimization of MALDI-TOF-MS, Sigma Xi Postdoctoral Poster Presentations, 2004, Undefined (Accessed March 24, 2025)