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Short-Chained Oligo(ethylene oxide)-Functionalized Gold Nanoparticles: Realization of Significant Protein Resistance



Kathryn Riley, Christopher Sims, Imani Wood, David J. Vanderah, Marlon L. Walker


Protein corona formed on nanomaterial surfaces play an important role in the bioavailability and cellular uptake of nanomaterials. Modification of surfaces with oligoethylene glycols (OEG) are a common way to improve the resistivity of nanomaterials to protein adsorption. Short-chain ethylene oxide (EO) oligomers have been shown to improve the protein resistance of planar Au surfaces. We describe the application of these EO oligomers for improved protein resistance of 30 nm spherical gold nanoparticles (AuNPs). Functionalized AuNPs were characterized using UV-Vis spectroscopy, dynamic light scattering (DLS), and zeta potential measurements. Capillary electrophoresis (CE) was used for separation and quantitation of AuNPs and AuNP-protein mixtures. Specifically, nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) was employed for the determination of equilibrium and rate constants for binding between citrate-stabilized AuNPs and two model proteins, lysozyme and fibrinogen. Semi-quantitative CE analysis was carried out for mixtures of EO-functionalized AuNPs and proteins, and results demonstrated a 2.5-fold to 10-fold increase in protein binding resistance to lysozyme depending on the AuNP surface functionalization and a 15-fold increase in protein binding resistance to fibrinogen for both EO oligomers examined in this study.
Analytical and Bioanalytical Chemistry


protein corona, gold nanoparticles, ethylene oxide, binding constant, capillary electrophoresis, NECEEM


Riley, K. , Sims, C. , Wood, I. , Vanderah, D. and Walker, M. (2017), Short-Chained Oligo(ethylene oxide)-Functionalized Gold Nanoparticles: Realization of Significant Protein Resistance, Analytical and Bioanalytical Chemistry, [online], (Accessed May 21, 2024)


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Created October 29, 2017, Updated October 12, 2021