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Shape Selectivity in Reversed-Phase Liquid Chromatography



Katrice Lippa, Kate Rimmer, Lane C. Sander


The separation of shape-constrained solutes in reversed-phase liquid chromatography is generally controlled by subtle differences in molecular shape together with stationary phase and operational parameters that promote stationary phase conformational order. This book chapter will examine the wealth of chromatographic and spectroscopic investigations that elucidate the dominant factors that control such shape selective processes. The review will center on alkyl chain-based chromatographic materials that have been utilized for the reversed-phase mode separation of solutes that differ only in molecular shape. Other shape recognition chromatographic materials such as molecular imprinted polymers (MIPs) and chiral stationary phases that may involve additional interactions (H-bonding, ion exchange) in the retentive process will not be reviewed. The various stationary phase properties and experimental conditions that influence chromatographic shape recognition will be discussed. Experimental evidence of stationary phase conformational order and disorder via spectroscopy investigations as well as complementary evidence provided through molecular simulations will be examined and related to the shape discrimination capability of shape-selective chromatographic sorbents. Lastly, the information provided herein will be used to propose plausible mechanisms of retention that may advance our current understanding of the shape-selective process in RPLC.
Shape Selectivity in Reversed-Phase Liquid Chromatography
Publisher Info
Book Chapter Book Chapter for Advances in Chromatography Book Series (2007),


alkyl chain conformation, alkyl-modified surface, column selectiity, monomeric, octadecylsilande stationary phase, polymeric


Lippa, K. , Rimmer, K. and Sander, L. (2008), Shape Selectivity in Reversed-Phase Liquid Chromatography, Book Chapter Book Chapter for Advances in Chromatography Book Series (2007), (Accessed July 14, 2024)


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Created October 16, 2008