U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Segmentation and Cell Tracking of Breast Cancer Cells

Published

Author(s)

Adele P. Peskin, Daniel J. Hoeppner, Christina H. Stuelten

Abstract

We describe a new technique to segment and track the cell images of a breast cancer cell line in order to study cell migration and metastasis. Within each image cell phenotypes vary widely, ranging from very bright completely bounded cells to barely visible cells with little to no apparent boundaries. A series of different steps defines the different cell morphologies. Cell segmentation and cell tracking are done simultaneously. Several new methods are presented for removing background intensity, and defining the presence of barely visible cells, including a new method for background subtraction and the use of selective dilation to define brighter cell areas. We show results for initial cell growth.
Citation
Cancer Science
Pub Type
Journals

Keywords

cancer cells, cell tracking, segmentation
Bioscience and Health

Citation

Peskin, A. , Hoeppner, D. and Stuelten, C. (2011), Segmentation and Cell Tracking of Breast Cancer Cells, Cancer Science (Accessed December 12, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created September 26, 2011, Updated February 19, 2017