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Displaying 26 - 50 of 1227

Mechanism of Action, Potency and Efficacy: Considerations for Cell and Gene Therapies

April 1, 2024
Author(s)
Carl Simon Jr., Anne L. Plant, Catherine Zander, Erich Bozenhardt, Christina Celluzzi, David Dobnik, Melanie Grant, John Hughes, Uma Lakshmipathy, Kok-Seong Lim, Laura Montgomery, Hesham Nawar, Thiana Nebel, Linda Peltier, James Sherley, Rouzbeh Taghizadeh, Eddie Tan, Sandrine Vessillier
One of the most challenging aspects of cell and gene therapy products (CGTPs) is defining mechanism of action (MOA), potency and efficacy of the product. This perspective examines these concepts and presents helpful ways to think about them through the

Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19

April 1, 2024
Author(s)
Jerilyn Izac, Edward Kwee, Adolfas Gaigalas, Lili Wang
COVID-19 is a global pandemic caused by the highly infectious SARS-CoV-2 virus. Efforts to combat SARS-CoV-2 infection include mass vaccination and development of monoclonal and convalescent plasma therapeutics that require precise measurements of

Comparison of N-glycopeptide to released N-glycan abundances and the influence of glycopeptide mass and charge state on N-linked glycosylation of IgG antibodies

March 7, 2024
Author(s)
Concepcion Remoroza, Meghan Burke Harris, Tytus Mak, Sergey Sheetlin, Yuri Mirokhin, Zachary Goecker, Brian T. Cooper, Mark Lowenthal, Xiaoyu (Sara) Yang, Guanghui Wang, Dmitrii V. Tchekhovskoi, Stephen E. Stein
We report the comparison of mass-spectral-based abundances of tryptic glycopeptides to fluorescence abundances of released labeled glycans and the effects of mass and charge state and in-source fragmentation on glycopeptide abundances. The primary

Digital PCR for the Characterization of Reference Materials

February 14, 2024
Author(s)
Megan Cleveland, Hua-Jun He, Mojca Milavec, Young-Kyung Bae, Peter Vallone, Jim Huggett
Well characterized reference materials are essential to ensuring the harmonization and accuracy of nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non

Heart-on-a-Chip Systems: Disease Modeling and Drug Screening Applications

February 14, 2024
Author(s)
Derrick Butler, Darwin Reyes-Hernandez
Cardiovascular disease (CVD) is the leading cause of death worldwide, casting a substantial economic footprint and burdening the global healthcare system. Historically, pre-clinical CVD modeling and therapeutic screening has been performed using animal

Establishing a framework for best practices for quality assurance and quality control in untargeted metabolomics

February 12, 2024
Author(s)
Tracey Schock, Jonathan Mosley, Chris Beecher, Warwick Dunn, Julia Kuligowski, Matthew Lewis, Georgios Theodoridis, Candice Ulmer, Dajana Vuckovic, Ian Wilson, Krista Zanetti
Background: Quality assurance (QA) and quality control (QC) practices are key tenets that facilitate study and data quality across all applications of untargeted metabolomics. Such important practices will strengthen this field and accelerate its success

SAND: automated time-domain modeling of NMR spectra applied to metabolite quantification

January 25, 2024
Author(s)
Yue Wu, Omid Sanati, Mario Uchimiya, Krish Krisnamurthy, Jonathan Wedell, Jeffrey C. Hoch, Arthur S. Edison, Frank Delaglio
Developments in untargeted nuclear magnetic resonance (NMR) metabolomics enable the profiling of thousands of bio-logical samples. Exploitation of this rich source of information requires detailed quantification of spectral features. How-ever, the

Standardization of Flow Cytometric Detection of Antigen Expression

January 25, 2024
Author(s)
Linhua Tian, Aaron Nelson, Tyler Lowe, Linda Weaver, Constance Yuan, Paul C. DeRose, Maryalice Stetler-Stevenson, Lili Wang
Background: While response to antigen-based immunotherapy relies upon the level of antigen expression by tumor cells and as decreasing levels of antigen expression can be an early indicator of developing resistance to therapy, we have developed an antigen

Editorial: Methods in Computational Genomics

January 24, 2024
Author(s)
Lei Chen, Nathanael David Olson
In the rapidly evolving field of Methods in Computational Genomics this editorial series illuminates the forefront of experimental techniques and methodologies. From dissecting large multidimensional numeric datasets to predicting the functions of novel

Quantification of mRNA in Lipid Nanoparticles Using Mass Spectrometry

December 26, 2023
Author(s)
Mark Lowenthal, Abigail Antonishek, Karen W. Phinney
Lipid nanoparticle-encapsulated messenger RNA (LNP-mRNA) holds great promise as a novel modality for treating a broad range of diseases. The ability to quantify mRNA accurately in therapeutic products helps to ensure consistency and safety. Here we

Cybersecurity of Genomic Data

December 20, 2023
Author(s)
Ronald Pulivarti, Natalia Martin, Frederick R. Byers, Justin Wagner, Justin Zook, Samantha Maragh, Jennifer McDaniel, Kevin Wilson, Martin Wojtyniak, Brett Kreider, Ann-Marie France, Sallie Edwards, Tommy Morris, Jared Sheldon, Scott Ross, Phillip Whitlow
Genomic data has enabled the rapid growth of the U.S. bioeconomy and is valuable to the individual, industry, and government because it has multiple intrinsic properties that in combination make it different from other types of high value data which

An investigation into an approach for automated supply chain onboarding

November 21, 2023
Author(s)
Elena Jelisic, Nenad Ivezic, Boonserm Kulvatunyou, Perawit Charoenwut, Ana Nikolov
The COVID-19 pandemic has brought the cold chain for bio-pharmaceutical products to the spotlight. To preserve their structure and function, biopharmaceuticals such as cells, proteins and enzymes, RNA molecules, and RNA-based drugs (e.g., mRNA-based COVID

Monitoring Spectrometer Drift with Unsupervised Machine Learning

November 20, 2023
Author(s)
Benjamin Harding, Ziling Hu, Ashley Hiett, Frank Delaglio, Katherine Henzler- Wildman, Chad Rienstra
Solid-state NMR spectroscopy (SSNMR) is a powerful technique to probe structural and dynamic properties of molecules at an atomic level. Modern SSNMR methods employ multidimensional pulse sequences requiring data collection over a period of days to weeks

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

November 18, 2023
Author(s)
Karina Torres Castro, Katherine Acuna-Umana, Leonardo Lesser-Rojas, Darwin Reyes-Hernandez
Blood is a complex sample comprised mostly of plasma, red blood cells (RBCs), and other cells whose concentrations correlate to physiological or pathological health conditions. There are also many blood-circulating biomarkers, such as circulating tumor

Metabolomics 2022 Workshop Report: State of QA/QC Best Practices in LC-MS-Based Untargeted Metabolomics, Informed Through mQACC Community Engagement Initiatives

November 8, 2023
Author(s)
Tracey Schock, Warwick Dunn, Julia Kuligowski, Jonathan Mosley, Candice Ulmer Holland, Dajana Vuckovic
Introduction: The Metabolomics Quality Assurance and Quality Control Consortium (mQACC) organized a workshop during the Metabolomics 2022 conference. Objectives: The goal of the workshop was to disseminate recent findings from mQACC community-engagement

Activation loop plasticity and active site coupling in the MAP kinase, ERK2

October 6, 2023
Author(s)
Laurel Pegram, Demian Riccardi, NATALIE AHN
Previous studies of the protein kinase, ERK2, using NMR and hydrogen-exchange measurements have shown changes in dynamics accompanying its activation by phosphorylation. However, knowledge about the conformational motions involved is incomplete. Here, we

Towards Ontologizing a Digital Twin Framework for Manufacturing

September 21, 2023
Author(s)
Milos Drobnjakovic, Guodong Shao, Ana Nikolov, Boonserm Kulvatunyou, Simon P. Frechette, Vijay Srinivasan
This paper presents a timely opportunity to ontologize a digital twin frame-work for manufacturing using recently published international standards and proposes a collaborative research effort to accomplish this task. The ISO/IEC 21838 series of standards
Displaying 26 - 50 of 1227