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Remineralization Effects of A Two-Solution Fluoride Mouthrinse: An in situ Study



Laurence C. Chow, Shozo Takagi, Clifton M. Carey, B A. Sieck


Results from previous studies showed that a two-component fluoride (F) rinse is significantly more effective than a NaF rinse of the same F content 250 g/g (ppm) in remineralizing enamel and root lesions in an in vitro cyclic de- and remineralization model. In the present study the two-solution rinse and two NaF rinses with F contents of 250 ppm and 1000 ppm were evaluated in an intraoral remineralization model. Caries-like lesions were formed in the enamel of extracted human molars with the use of a pH 4 demineralizing solution. Thin sections of the({approximately}120 mm) containing lesions were prepared, and the mineral contents of the lesions were assessed by quantitative micro-radiography. With the cut surfaces protected by nail varnish, the sections in 3 groups were mounted with wax in the lingual areas of a removable mandibular appliance. Each of the seven subjects wore such an appliance for 14 days continuously except during subject's eating, drinking and carrying out oral hygiene procedures. Twice daily (after breakfast and before bedtime) the subjects received a 1 min rinse with 20 mL of (1) 250 ppm F NaF rinse, (2) 1000 ppm F NaF rinse, or (3) 228 ppm F two-component F rinse. At the end of the experimental period, the sections were retrieved, and the mineral contents of the lesions were again assessed quantitatively. The results show that both the 1000 ppm NaF and 228 ppm two-solution rinses were more effective (p 0.05) to that produced by the NaF rinse with 4 times the F content (1000 ppm). These results suggest that highly effective low F dose rinses or dentifrice's can be formulated.
Journal of Dental Research


calcium fluoride, enamel, fluoride, fluoride rinse, in situ model, remineralization, sodium hexafluorosilicate


Chow, L. , Takagi, S. , Carey, C. and Sieck, B. (1999), Remineralization Effects of A Two-Solution Fluoride Mouthrinse: An in situ Study, Journal of Dental Research, [online], (Accessed July 13, 2024)


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Created October 1, 1999, Updated February 19, 2017