Quantitative Assessment of Nanoparticle Induced Oxidative Damage to DNA Using Isotope Dilution Mass Spectrometry

Published

June 16, 2014

Author(s)

Elijah J. Petersen, Pawel Jaruga, M Miral Dizdar, Bryant C. Nelson

Abstract

Mass spectrometry techniques have been employed as one of the primary analytical tools for investigating the effects of oxygen-derived free radicals on the induction and subsequent repair of oxidatively-induced DNA damage in living systems. The National Institute of Standards and Technology has established a comprehensive research program focused on identifying mechanisms of DNA damage caused by commercially relevant engineered nanoparticles (NPs) using high resolution mass spectrometry for the quantification of oxidatively-induced DNA damage. We present results from recently published studies on the genotoxicity of National Institute of Standards and Technology (NIST) reference material (RM) gold nanoparticles (AuNPs) and on DNA damage in C. elegans after exposure to 10 nm silver nanoparticles (AgNPs).

Proceedings Title

Nanotech 2014

Conference Dates

June 16-18, 2014

Conference Location

Washington, DC

Pub Type

Conferences

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Keywords

nanotoxicology, Comet assay, genotoxicity, biomarker, toxicology

Citation

Petersen, E. , Jaruga, P. , , M. and Nelson, B. (2014), Quantitative Assessment of Nanoparticle Induced Oxidative Damage to DNA Using Isotope Dilution Mass Spectrometry, Nanotech 2014, Washington, DC, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=915778 (Accessed October 2, 2025)