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Protein labeling in Escherichia coli with 2H, 13C and 15N

Published

Author(s)

Zvi Kelman, Prasad T. Reddy

Abstract

A number of structural biology techniques such as nuclear magnetic resonance (NMR) and small-angle neutron scattering (SANS) can be performed with proteins with nuclei at natural isotope abundance. However, the use of proteins labeled with heavy isotopes (2H, 13C and 15N) enabled greater measurements flexibility. In this chapter several methods for uniform and fractional protein labeling with heavy isotopes using Escherichia coli in a defined media are described. The methods described can be used for labeling with single or multiple isotopes.
Citation
Isotope labeling of biomolecules
Volume
565
Publisher Info
Elsevier, New York New York, NY

Keywords

13C, deuterium, E. coli, 2H, 15N, protein expression, stable isotope labeling

Citation

Kelman, Z. and Reddy, P. (2015), Protein labeling in Escherichia coli with 2H, 13C and 15N, Isotope labeling of biomolecules, Elsevier, New York New York, NY, [online], https://doi.org/10.1016/bs.mie.2015.08.023 (Accessed July 23, 2024)

Issues

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Created August 23, 2015, Updated November 10, 2018