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Protein Dynamics in Viscous Solvents



G Caliskan, A Kisliuk, A M. Tsai, Christopher Soles, Alexei Sokolov


The principals governing the effective stabilization of tissues and drugs are unclear and effective agents for preservation are discovered largely by trail and error. We explore the use of low frequency Raman spectroscopy as an effective tool fro direct evaluating the ability of different solvents to suppress the conformational fluctuations that can lead to both protein activity and denaturation. We demonstrate that while trehalose provides superior stabilization at high temperatures, glycerol is more effective at suppressing protein dynamics at low temperatures. The presented results demonstrate that viscosity of the solvent is not the only parameter important for biopreservation.
Journal of Chemical Physics
No. 9


cryopreservation, glycerol, lysozyme, myoglobin, protein stability, Raman scattering, trehalose


Caliskan, G. , Kisliuk, A. , Tsai, A. , Soles, C. and Sokolov, A. (2003), Protein Dynamics in Viscous Solvents, Journal of Chemical Physics (Accessed June 22, 2024)


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Created February 28, 2003, Updated October 12, 2021