Protein Dynamics in Viscous Solvents

Published

March 1, 2003

Author(s)

G Caliskan, A Kisliuk, A M. Tsai, Christopher Soles, Alexei Sokolov

Abstract

The principals governing the effective stabilization of tissues and drugs are unclear and effective agents for preservation are discovered largely by trail and error. We explore the use of low frequency Raman spectroscopy as an effective tool fro direct evaluating the ability of different solvents to suppress the conformational fluctuations that can lead to both protein activity and denaturation. We demonstrate that while trehalose provides superior stabilization at high temperatures, glycerol is more effective at suppressing protein dynamics at low temperatures. The presented results demonstrate that viscosity of the solvent is not the only parameter important for biopreservation.

Citation

Journal of Chemical Physics

Volume

118

Issue

No. 9

Pub Type

Journals

Keywords

cryopreservation, glycerol, lysozyme, myoglobin, protein stability, Raman scattering, trehalose

Citation

Caliskan, G. , Kisliuk, A. , Tsai, A. , Soles, C. and Sokolov, A. (2003), Protein Dynamics in Viscous Solvents, Journal of Chemical Physics (Accessed July 15, 2025)