Protective roles of single-wall carbon nanotubes in ultrasonication-induced DNA base damage
Elijah J. Petersen, Xiaomin X. Tu, M Miral Dizdar, Ming Zheng, Bryant C. Nelson
Ultrasonication is a physical process known to generate abundant levels of oxygen-derived species such as hydroxyl radicals, hydrogen atoms, and hydrogen peroxide. Highly reactive hydroxyl radicals can react with DNA to generate strand breaks and a multiplicity of oxidatively modified DNA bases. Oxidatively induced damage to DNA bases has been well-studied and the reaction mechanisms have been resolved. Gas chromatography-mass spectrometry (GC-MS) methodologies have been developed to both qualitatively and quantitatively measure the DNA lesions. Here, we investigated DNA base damage in ATT and GT oligomers from ultrasonication in the presence and absence of sin-gle-wall carbon nanotubes (SWCNTs). We found that the overall level of DNA damage is reduced in the presence of SWCNTs, particularly for DNA lesions formed by one-electron reduction of intermediate radicals. The protective role of SWCNTs observed in this work suggests a contrary view to the general idea that CNTs interact negatively with bio-molecules.
, Tu, X.
, , M.
, Zheng, M.
and Nelson, B.
Protective roles of single-wall carbon nanotubes in ultrasonication-induced DNA base damage, Journal of the American Chemical Society, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=911310
(Accessed November 28, 2023)