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Portable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals



Aleksey Yevtodiyenko, Arkadiy Bazhin, Pavlo Khodakivskyi, Aurelien Godinat, Gyslain Budin, Marina Kunchulia, Giorgio Pietramaggiori, Sandra Scherer, Elena Goun, Sergey Polyakov, George P. Eppeldauer


Bioluminescent imaging (BLI) is one of the most powerful and widely used preclinical imaging modalities. However, the current technology relies on the use of transgenic luciferase-expressing cells and animals and therefore can only be applied to a limited number of existing animal models of human disease. Here, we report the development of a "portable bioluminescent" (PBL) technology that overcomes most of the major limitations of traditional BLI. We demonstrate that the PBL method is capable of noninvasive measuring the activity of both extracellular (e.g., dipeptidyl peptidase 4) and intracellular (e.g., cytochrome P450) enzymes in vivo in non-luciferase-expressing mice. Moreover, we successfully utilize PBL technology in dogs and human cadaver, paving the way for the translation of functional BLI to the noninvasive quantification of biological processes in large animals. The PBL methodology can be easily adapted for the noninvasive monitoring of a plethora of diseases across multiple species.
Nature Communications


Bioluminescence imaging, portable light detection, noninvasive measurements, faint light


Yevtodiyenko, A. , Bazhin, A. , Khodakivskyi, P. , Godinat, A. , Budin, G. , Kunchulia, M. , Pietramaggiori, G. , Scherer, S. , Goun, E. , Polyakov, S. and Eppeldauer, G. (2021), Portable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals, Nature Communications, [online],, (Accessed July 24, 2024)


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Created May 11, 2021, Updated October 12, 2021