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Novel Detection Method for DNA Hybridization Based on Molecular Spotlighting

Published

Author(s)

T H. Huang, Michael J. Tarlov

Abstract

A new platform for monitoring hybridization of thiol-derivatized DNA probes (SH-DNA) immobilized on gold surfaces is currently being developed. The gold surface is modified with mixed monolayers of the SH-DNA probes and 6-mercaptohexanol. In fluorescence-based detection scheme, the fluorophore is typically attached to the target DNA. In our investigation, the probe DNA is tagged with the fluorophore instead. The detection principle is based on the dramatic structural changes between single- (ss-) and double-stranded (ds-) DNAs. Prior to hybridization, the fluorophore is expected to be in close proximity to the gold surface, resulting in the quenching of the fluorescence signal. Upon hybridization, the dsDNA adopts a rod-like structure that extends the fluorophore away from the gold surface. With the fluorphores located further from the gold surface, quenching is reduced and an increase in fluorescence intensity is observed. Parameters that affect fluorescence signal are examined and discussed.
Proceedings Title
Sigma Xi Post Doctoral Poster Presentations, 2000
Conference Dates
February 17-18, 2000
Conference Title
Sigma Xi Web Page

Keywords

DNA, DNA hybridization, molecular spotlighting, thiol-derivatized DNA probes

Citation

Huang, T. and Tarlov, M. (2000), Novel Detection Method for DNA Hybridization Based on Molecular Spotlighting, Sigma Xi Post Doctoral Poster Presentations, 2000 (Accessed July 24, 2024)

Issues

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Created February 1, 2000, Updated February 17, 2017