Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Nonuniform Sampling in Multidimensional NMR for Improving Spectral Sensitivity



Matthew Zambrello, Adam D. Schuyler, Mark W. Maciejewski, Frank Delaglio, Irina Bezsonova, Jeffrey C. Hoch


The development of multidimensional NMR spectroscopy enabled an explosion of structural and dynamical investigations on proteins and other biomacromolecules. Practical limitations on data sampling, based on the Jeener paradigm of parametric sampling of indirect time domains, have long placed limits on resolution in the corresponding frequency dimensions. The emergence of nonuniform sampling (NUS) in indirect time dimensions circumvents those limitations, affording high resolution spectra from short data records collected in practically realized measurement times. In addition to substantially improved resolution, NUS can also be exploited to improve sensitivity, with gains comparable to those obtained using cryogenically cooled probes. We describe a general approach for acquiring and processing multidimensional NUS NMR data for improving sensitivity.


NMR, Non-Uniform Sampling, Spectral Processing


Zambrello, M. , Schuyler, A. , Maciejewski, M. , Delaglio, F. , Bezsonova, I. and Hoch, J. (2018), Nonuniform Sampling in Multidimensional NMR for Improving Spectral Sensitivity, Methods, [online], (Accessed April 18, 2024)
Created March 31, 2018, Updated October 12, 2021