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New perspective on glycoside hydrolase binding to lignin from pretreated corn stover



John M. Yarbrough, Ashutosh Mittal, Elisabeth Mansfield, Larry Taylor, Sarah Hobdey, Deanne W. Sammond, Yannick J. Bomble, Michael F. Crowley, Stephen R. Decker, Michael E. Himmel, Todd B. Vinzant


Non-specific binding of cellulases to lignin has been implicated as a major factor in the loss of cellulase activity during biomass conversion to sugars. It is believed that this binding may strongly impact process economics through loss of enzyme activities during hydrolysis and enzyme recycling scenarios. The current model suggests glycoside hydrolase activities are lost though non-specific/non-productive binding of carbohydrate-binding domains to lignin, limiting catalytic site access to the carbohydrate components of the cell wall.
Biotechnology for Biofuels


biofuels, lignin


Yarbrough, J. , Mittal, A. , Mansfield, E. , Taylor, L. , Hobdey, S. , Sammond, D. , Bomble, Y. , Crowley, M. , Decker, S. , Himmel, M. and Vinzant, T. (2015), New perspective on glycoside hydrolase binding to lignin from pretreated corn stover, Biotechnology for Biofuels, [online],, (Accessed April 18, 2024)
Created December 17, 2015, Updated October 12, 2021