A new approach to quantification of mAb aggregates using peptide affinity probes

Published

February 10, 2017

Author(s)

Crystal Cheung, Kyle Anderson, Pooja Patel, Keale Cade, Karen W. Phinney, Illarion Turko

Abstract

Using mAbs as therapeutic molecules is complicated by the propensity of mAbs to aggregate at elevated concentrations, which can lead to a variety of adverse events in treatment. Here, we describe a proof-of-concept for new methodology to detect and quantify mAb aggregation. Assay development included using an aggregated mAb as bait for screening of phage display peptide library and identifying those peptides with random sequence which can recognize mAb aggregates. Once identified, the selected peptides can be used for developing quantitative methods to assess mAb aggregation. Results indicate that a peptide binding method coupled with mass spectrometric detection of bound peptide can quantify mAb aggregation and potentially be useful for monitoring aggregation propensity of therapeutic protein candidates.

Citation

Nature - Scientific Reports

Pub Type

Journals

Download Paper

https://doi.org/10.1038/srep42497

Local Download

Keywords

mAbs, aggregation, NISTmAb, quantification, mass spectrometry, peptide phage display

Citation

Cheung, C. , Anderson, K. , Patel, P. , Cade, K. , Phinney, K. and Turko, I. (2017), A new approach to quantification of mAb aggregates using peptide affinity probes, Nature - Scientific Reports, [online], https://doi.org/10.1038/srep42497, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=921361 (Accessed April 18, 2024)