U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Myristoylation Restricts Orientation of the GRASP Domain on Membranes and Promotes Membrane Tethering

Published

Author(s)

Frank N. Heinrich, Hirsh Nanda, Goh Haw Zan, Collin Bachert, Mathias Loesche, Adam D. Linstedt

Abstract

The mammalian GRASP proteins are Golgilocalized homotypic membrane tethers that organize Golgi stacks into a long, contiguous ribbon-like structure. It is unknown how GRASPs undergo trans pairing given that cis interactions between the proteins in the plane of the membrane are intrinsically favored. To test the hypothesis that myristoylation of the selfinteracting GRASP domain restricts its orientation on the membrane to favor trans pairing we established an in vitro assay that recapitulates GRASP-dependent membrane tethering and used neutron reflection under these same conditions to determine the orientation of the GRASP domain. In vivo, the membrane association of GRASP proteins is conferred by the simultaneous insertion of an N-terminal myristic acid and binding to a Golgi-associated binding partner. In our assay, the latter contact was replaced using a C-terminal hexa-His moiety, which bound to Ni2+-conjugated lipids incorporated into a substrate-supported bilayer lipid membrane. Non-myristoylated protein lacked a fixed orientation on the membrane and inefficiently tethered liposomes. In contrast, myristoylated GRASP promoted tethering and exhibited a unique membrane complex. Thus, myristoylation restricts the membrane orientation of the GRASP domain favoring interactions in trans for membrane tethering.
Citation
Journal of Biological Chemistry
Pub Type
Journals

Download Paper

DOI Link

Keywords

Golgi organization, protein-membrane complex, neutron reflection
Neutron research

Citation

Heinrich, F. , Nanda, H. , Zan, G. , Bachert, C. , Loesche, M. and Linstedt, A. (2014), Myristoylation Restricts Orientation of the GRASP Domain on Membranes and Promotes Membrane Tethering, Journal of Biological Chemistry, [online], https://doi.org/10.1074/jbc.m113.543561 (Accessed February 15, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created April 4, 2014, Updated May 9, 2022