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Microstructures of Collagen-Hyaluronic Acid Hydrogels by Two-Photon Fluorescence Microscopy



SK Park, S E. Wu, C A. Khatri, H Suh, Francis W. Wang


Scanning electron microscopy (SEM) and histological techniques have often been used to determine the microstructures of collagen-based hydrogels. However, these techniques entail cross-sectioning, dehydration and fixation procedures that inevitably affect the morphology of a hydrogel. Confocal fluorescence microscopy is of advantage to SEM because the only sample preparation required is the conjugation of a fluorescent dye to the polymer segments in a hydrogel. Two-photon fluorescence microscopy (TPFM) is an excellent method for cell or tissue imaging because of its inherent 3D resolution and long penetration depth. In addition, this method provides biochemical information about cells or tissues and causes minimal photodamage. An application of TPFM to the characterization of the microstructures of collagen-hyaluronic acid (HA) hydrogels is described in this paper.
World Biomaterials Congress


collagen, confocal microscopy, focal adhesion, hyaluronic acid, hydrogels


Park, S. , Wu, S. , Khatri, C. , Suh, H. and Wang, F. (2004), Microstructures of Collagen-Hyaluronic Acid Hydrogels by Two-Photon Fluorescence Microscopy, World Biomaterials Congress, [online], (Accessed June 18, 2024)


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Created December 31, 2003, Updated October 12, 2021