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Microfluidic Temperature Gradient Focusing

Published

Author(s)

David J. Ross, Laurie E. Locascio

Abstract

A new technique is described for the concentration and separation of ionic species in a microchannels. Concentration is achieved by balancing the electrophoretic velocity of an analyte against the bulk flow of solution in the presence of a temperature gradient. With an appropriate buffer, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity, so that the electrophoretic and bulk velocities sum to zero at a unique point, resulting in analyte focusing. The technique for a variety of analytes including, amino acids, DNA, proteins, and particles, is shown to be capable of greater than 10000-fold concentration.
Citation
Analytical Chemistry
Volume
74
Issue
No. 11

Keywords

focusing, microfluidics, preconcentration

Citation

Ross, D. and Locascio, L. (2002), Microfluidic Temperature Gradient Focusing, Analytical Chemistry (Accessed July 18, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created June 1, 2002, Updated February 17, 2017