Skip to main content
U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Measuring silver nanoparticle dissolution in complex biological and environmental matrices using UV/visible absorbance measurements



Justin M. Zook, Robert MacCuspie, Stephen E. Long, Elijah J. Petersen


This special publication is one in a series stemming from the National Nanotechnology Initiative (NNI) Nano-EHS Research Strategy which identified Nanomaterial Measurement Infrastructure as one of the essential areas of research needed in order to develop an effective risk assessment and management plan regarding various aspects of nanotechnology in consumer products as it pertains to human health, exposure and the environment. The National Institute of Standards and Technology (NIST) was identified as a lead agency in the development of measurement strategies for the robust development to assess the potential effects of engineered nanomaterials and their fate in the environment. One important factor in these measurements is a reliable method to measure changes to engineered nanomaterials, such as particle dissolution, in biologically and environmentally relevant matrices. The current protocol presents a method to measure dissolution of silver nanoparticles in biological and environmental matrices by measuring changes to the dispersions using UV-vis absorbance measurements. Updates to this protocol may be released in the future. Visit measurement-protocols.cfm to check for revisions of this protocol, or new protocols in the series. We also encourage users to report citations to published work in which this protocol has been applied.
Special Publication (NIST SP) - 1200-13
Report Number
Created June 3, 2015, Updated November 10, 2018