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Measuring Cell Adhesion and Proliferation in Polymer Scaffolds by X-Ray Microcomputed Tomography

Published

Author(s)

Shauna M. Dorsey, Sheng Lin-Gibson, Carl Simon Jr.

Abstract

Statement of Purpose: We have investigated the ability of X-ray microcomputed tomography (CT) to make three-dimensional (3D) measurements of cell adhesion and proliferation in polymeric tissue engineering scaffolds [1]. Standard methods for measuring cell number in scaffolds include fluorescence microscopy and soluble assays for enzymes, protein or DNA. Microscopy yields two-dimensional images of cell distribution but is not quantitative. Confocal microscopy can generate 3D images of cell distribution, but cannot penetrate opaque scaffold struts to image the interior of scaffolds. Soluble assays are quantitative but do not yield images of cell distribution. In contrast, CT generates 3D images, can penetrate deep into the scaffold interior and is quantitative. For these reasons, we have investigated CT for imaging cells in tissue scaffolds.
Proceedings Title
society for biomaterials annual meeting
Conference Dates
April 22-25, 2009
Conference Location
San Antonio, TX, US

Keywords

x-ray microcomputed tomography, 3d imaging, cell proliferation, polymer scaffold, cell adhesion

Citation

Dorsey, S. , Lin-Gibson, S. and Simon Jr., C. (2009), Measuring Cell Adhesion and Proliferation in Polymer Scaffolds by X-Ray Microcomputed Tomography, society for biomaterials annual meeting, San Antonio, TX, US, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=900246 (Accessed May 26, 2024)

Issues

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Created April 29, 2009, Updated October 12, 2021