NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Measuring Cell Adhesion and Proliferation in Polymer Scaffolds by X-Ray Microcomputed Tomography

Published

Author(s)

Shauna M. Dorsey, Sheng Lin-Gibson, Carl Simon Jr.

Abstract

Statement of Purpose: We have investigated the ability of X-ray microcomputed tomography (CT) to make three-dimensional (3D) measurements of cell adhesion and proliferation in polymeric tissue engineering scaffolds [1]. Standard methods for measuring cell number in scaffolds include fluorescence microscopy and soluble assays for enzymes, protein or DNA. Microscopy yields two-dimensional images of cell distribution but is not quantitative. Confocal microscopy can generate 3D images of cell distribution, but cannot penetrate opaque scaffold struts to image the interior of scaffolds. Soluble assays are quantitative but do not yield images of cell distribution. In contrast, CT generates 3D images, can penetrate deep into the scaffold interior and is quantitative. For these reasons, we have investigated CT for imaging cells in tissue scaffolds.
Proceedings Title
society for biomaterials annual meeting
Conference Dates
April 22-25, 2009
Conference Location
San Antonio, TX, US
Pub Type
Conferences

Download Paper

Local Download

Keywords

x-ray microcomputed tomography, 3d imaging, cell proliferation, polymer scaffold, cell adhesion
Biomaterials

Citation

Dorsey, S. , Lin-Gibson, S. and Simon Jr., C. (2009), Measuring Cell Adhesion and Proliferation in Polymer Scaffolds by X-Ray Microcomputed Tomography, society for biomaterials annual meeting, San Antonio, TX, US, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=900246 (Accessed October 18, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created April 29, 2009, Updated October 12, 2021
Was this page helpful?