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Measurement of oxidatively modified DNA bases and nucleosides by isotope-dilution mass spectrometry

Published

Author(s)

Pawel Jaruga

Abstract

Among cellular structures, the genome is particularly prone to damage, which can result from spontaneous reactions, replication linked failures, or oxidative processes due to metabolic derivatives or to external agents. Damage to DNA causes more severe consequences than damage to replaceable cellular macromolecules because the genome must be preserved for the life of the cell, and because it can be copied and proliferated into next generations of the cells. Reactions of free radicals and other redox capable agents with DNA generate an abundance of products in nuclear and mitochondrial DNA of living organisms. Growing evidence points to the involvement of this type of damage in the etiology of numerous diseases including carcinogenesis. Comprehensive understanding of the mechanisms, cellular repair, and biological consequences of DNA damage requires accurate measurement of resulting products. There are various analytical techniques, with their own advantages and drawbacks, which can be used for this purpose. Mass spectrometric techniques with isotope dilution provide structural interpretation of products and accurate quantification, which ascertain reliable measurement. Gas chromatography-mass spectrometry or liquid chromatography-mass spectrometry, in single or tandem versions, have been used for the measurement of numerous DNA products such as sugar and base lesions, 8,5′-cyclopurine-2′-deoxynucleosides, base-base tandem lesions, and DNA-protein crosslinks, in vitro and in vivo. Basic concepts and results will be presented and discussed.
Proceedings Title
Journal of Chromatography & Separation Techniques
Volume
6
Issue
4
Conference Dates
July 12-15, 2015
Conference Location
New Orleans, LA
Conference Title
Current Trends in Mass Spectrometry

Keywords

oxidative DNA damage, isotope-dilution mass spectrometry, 8, 5¿-cyclopurine-2¿-deoxynucleosides

Citation

Jaruga, P. (2015), Measurement of oxidatively modified DNA bases and nucleosides by isotope-dilution mass spectrometry, Journal of Chromatography & Separation Techniques, New Orleans, LA, [online], https://doi.org/10.4172/2157-7064.S1.007 (Accessed April 14, 2024)
Created September 1, 2015, Updated January 27, 2020