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Measurement of oxidatively induced DNA damage and its repair by mas spectrometric techniques

Published

Author(s)

M Miral Dizdar, Erdem Coskun, Pawel Jaruga

Abstract

Oxidatively induced damage caused by free radicals and other DNA-damaging agents generate a plethora of products in DNA of living organisms. There is mounting evidence for the involvement of this type of damage in the etiology of numerous diseases including carcinogenesis. For thorough understanding of mechanisms, cellular repair and biological consequences of DNA damage, accurate measurement of resulting products must be achieved. There are various analytical techniques with their own advantages and drawbacks that can be used for this purpose. Mass spectrometric techniques with isotope-dilution that include gas chromatography and liquid chromatography provide structural elucidation of products and ascertains accurate quantification, which are absolutely necessary for reliable measurements. Both gas chromatography-mass spectrometry or liquid chromatography-mass spectrometry in single or tandem versions have been used for the measurement of numerous DNA products such as sugar and base lesions, 8,5'-cyclopurine-2'-deoxynucleosides, base-base tandem lesions and DNA-protein cross-links in vitro and in vivo. This article reviews these techniques and their applications to the measurement of oxidatively induced DNA damage and its cellular repair.
Citation
Free Radical Research
Volume
49
Issue
5

Keywords

free radicals, hydroxyl radical, 8, 5'-cyclopurine-2'-deoxynucleosides, DNA base damage, DNA sugar damage, gas chromatography-mass spectrometry, isotope-dilution, liquid chromatography-mass spectrometry.

Citation

, M. , Coskun, E. and Jaruga, P. (2015), Measurement of oxidatively induced DNA damage and its repair by mas spectrometric techniques, Free Radical Research, [online], https://doi.org/10.3109/10715762.2015.1014814 (Accessed October 26, 2021)
Created March 25, 2015, Updated January 27, 2020