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Measurement of Oxidative DNA Damage in the Human p53 and PGK1 Gene at Nucleotide Resolution

Published

Author(s)

S A. Akman, H Rodriguez

Abstract

Reactive oxygen species (ROS) from normal metabolism cause damage to DNA, protein and lipid (fat). By developing an oxidative DNA damage mapping version of the Ligation Mediated Polymerase Chain Reaction (LMPCR), we investigated the in vivo and in vitro frequencies of DNA base modifications caused by ROS in human p53 and PGK1 gene. In vivo and in vitro DNA base modification patterns were nearly identical. In human male fibroblasts, these patterns were independent of the transition metal used (Cu(II), Fe(II), or Cr(VI). Furthermore, the local probability of H202-mediated DNA base damage was determined primarily by DNA sequence context.
Citation
Annals Of The New York Academy Of Sciences

Keywords

DNA base damage, free radicals, ligation mediated polymerase chain react, LMPCR, mapping, oxidative stress

Citation

Akman, S. and Rodriguez, H. (2021), Measurement of Oxidative DNA Damage in the Human p53 and PGK1 Gene at Nucleotide Resolution, Annals Of The New York Academy Of Sciences (Accessed June 17, 2024)

Issues

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Created October 12, 2021