Measurement of Interglycosidic 3Jch Coupling Constants of Selectivity 13C Labled Oligosaccharides by 2D J-Resolved 1H NMR Spectroscopy

Published

February 9, 1998

Author(s)

V Poszgay, Fahriye N. Sari, Bruce Coxon

Abstract

Tri-, tetra-, penta- saccharide fragments of the O-polysaccharide of Shigella dysenteriae type 1 have been prepared in which a D-galactose residue of each oligosaccharide methyl glycoside derivative contains a ¹³C label at C-1. The interglycosidic coupling constants [³J_CH] of these ¹³C nuclei with the H-3 nuclei of the adjacent 2-acetamido-2-deoxy-D-glucose residues have been measured by two-dimensional, J-resolved ^IH NMR spectroscopy. The magnitudes of these coupling constants indicate that the trisaccharide is conformationally different to the higher oligosaccharide homologs, in agreement with previous studies of ¹³C chemical shifts and ¹J_CH values.

Citation

Carbohydrate Research

Volume

308

Pub Type

Journals

Keywords

2D J-resolved NMR spectroscopy, carbon-13 labeling, carbon-13--proton coupling constants, oligosaccharides, pentasaccharide, Shigella dysenteriae type 1, tetrasaccharide, trisachharides

Citation

Poszgay, V. , Sari, F. and Coxon, B. (1998), Measurement of Interglycosidic ³J_ch Coupling Constants of Selectivity ¹³C Labled Oligosaccharides by 2D J-Resolved ¹H NMR Spectroscopy, Carbohydrate Research (Accessed December 14, 2024)