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Mapping Oxidative DNA Damage at Nucleotide Resolution in Mammalian Cells



H Rodriguez, T R. O'Connor, H H. Chen, S M. Dai, S D. Flanagan, G P. Holmquist, S A. Akman, A D. Riggs


Analytical techniques including mass spectrometry have proven invaluable for the detection of DNA adducts, but these methods do not map the squence context dependent distribution of adducts. The adduct maps are important to understanding the formation of pre-mutagenic lesions that appear in critical growth control genes that are linked to cellular transformation (e.g. P53). In this chapter, we describe some of the methods available for the detection of oxidized base adducts at nucleotide resolution in human cells and some results obtained using this technology.
Oxidative Stress and Aging Journal


IMPCR, oxidative stress, reactive oxygen species, TDPCR


Rodriguez, H. , O'Connor, T. , Chen, H. , Dai, S. , Flanagan, S. , Holmquist, G. , Akman, S. and Riggs, A. (2008), Mapping Oxidative DNA Damage at Nucleotide Resolution in Mammalian Cells, Oxidative Stress and Aging Journal (Accessed February 26, 2024)
Created October 16, 2008