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Intrinsic Standards for UV/Visible Spectrophotometry



John C. Travis, David L. Duewer, Marc L. Salit


The 21st century global marketplace and international performance standards provide a steady demand for fit-for-purpose reference materials. An efficient system for the delivery of reference materials to end-users begins with responsive and competitive secondary standards producers with the ability to document traceability of their products. The number of links in the traceability chain may be significantly shortened by the availability of intrinsic (data) standards. An excellent example is provided by a solution of holmium oxide in dilute perchloric acid; with 18 UV/visible absorption bands whose peak minimum location is an intrinsic property of the solution. Certification of the holmium oxide solution bands as intrinsic standards packages the top links of the chain into a single-stage documentation of traceability to the meter. Secondary standards providers need only produce the solution according to instructions and seal it carefully in cuvettes or ampoules in order to assert traceability though the published and peer-reviewed intrinsic data.
American Laboratory


certified wavelengths, holmium oxide solution, intrinsic standards, molecular absorption, reference materials, spectral convolution, uv/visible spectrophotometry, wavelength standards


Travis, J. , Duewer, D. and Salit, M. (2004), Intrinsic Standards for UV/Visible Spectrophotometry, American Laboratory (Accessed May 26, 2024)


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Created April 1, 2004, Updated February 17, 2017