An improved method for calibrating solid-phase microextraction by direct loading
Mengyan Gong, Dustin Poppendieck
Solid-phase microextraction (SPME) has been widely used as a sampling technique for a variety of organic compounds due to its advantage of small sampling volume, ease of sampling, and simple injection (Ouyang and Pawliszyn 2008). Recently, Cao et al. (2016) has developed an improved method for measuring emission parameter for semivolatile organic compound (SVOC), which shortened experimental times. In the method by Cao et al. (2016), the SVOC equilibrium concentration in a sealed chamber was measured using SPME sampling. Hence, calibration of SPME is required. The calibration requires that the absolute amount of SVOC adsorption onto the SPME fiber to be measured. To do this, Cao et al. (2016) calibrated SPME using liquid splitless injection and assumed that the SPME injection and liquid splitless injection has the same transfer efficiency. However, it is known that the transfer efficiency for both SPME injection and splitless injection are influenced by factors such as type of liner, temperature of injector, and cross-sectional area of the space between column and liner, so that transfer efficiencies could be significantly different for the two injection techniques (Ouyang, Chen et al. 2005). Hence, calibrating SPME using liquid splitless injection may cause large uncertainties. Among the loading techniques that have been used for SPME, direct syringe-fiber loading has been shown to have transfer efficiencies greater than 95 % for chemicals with low volatility (Zhao, Ouyang et al. 2007). This study aims to first investigate the applicability of the syringe-fiber loading approach for calibrating SPME, and then applies it to experiments measuring the Tris(2-chloro-1-methylethy) Phosphate (TCPP) partition coefficient between spray polyurethane foam (SPF) and air based on the method by Cao et al. (2016).
and Poppendieck, D.
An improved method for calibrating solid-phase microextraction by direct loading, Healthy Buildings 2017 Asia, Tianan, TW, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=923169
(Accessed December 7, 2023)