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Hyperspectral Imaging of Ischemic Wounds



David W. Allen


Optical imaging has the potential to achieve high spatial resolution and high functional sensitivity in wound assessment. However, clinical acceptance of many optical imaging devices is hampered by poor reproducibility, low accuracy, and lack of biological interpretation. We developed an in vivo model of ischemic flap for non-contact assessment of wound tissue functional parameters and spectral characteristics. The model was created by elevating the bipedicle skin flaps of a domestic pig from the underlying vascular bed and inhibiting graft bed reperfusion by a silastic sheet. Hyperspectral imaging was carried out on the ischemic flap model and compared with transcutaneous oxygen tension and perfusion measurements at different positions of the wound. Hyperspectral images have also been captured continuously during a post-occlusive reactive hyperemia (PORH) procedure. Tissue spectral characteristics obtained by hyperspectral imaging correlated well with cutaneous tissue oxygen tension, blood perfusion, and microscopic changes of tissue morphology. Our experiments not only demonstrated the technical feasibility for quantitative assessment of chronic wound but also provided a potential digital phantom platform for quantitative characterization and calibration of medical optical devices.
Proceedings Title
Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of
Tissue IV
Conference Dates
January 21-26, 2012
Conference Location
San Francisco, CA
Conference Title
SPIE Photonics West BiOS 2011


cutaneous, hyperspectral imaging, ischemia, oxygenation, standardization


Allen, D. (2012), Hyperspectral Imaging of Ischemic Wounds, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV , San Francisco, CA, [online], (Accessed June 14, 2024)


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Created February 1, 2012, Updated November 10, 2018