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Hybridization Thermodynamics of Bound DNA

Published

Author(s)

Brian E. Lang

Abstract

Isothermal Titration Calorimetry was used to study the thermodynamics of hybridization on DNA-functionalized colloidal gold nanoparticles. When compared to the thermodynamics of hybridization of DNA that is free in solution, the differences in the values of rG°, rH° and rS° were minimal, and typically statistically insignificant. However, ITC was also to reveal information about the binding stoichiometry of the hybridization reactions on the DNA functionalized gold nanoparticles, and the experiments indicate that there is a significant fraction of the DNA on gold nanoparticle surface that is unavailable for DNA hybridization. Furthermore, the fraction of available DNA is also dependent on the spacer group on the DNA that is used to span the gold surface from that to the probe DNA.
Citation
Journal of the American Chemical Society
Volume
42

Keywords

Isothermal Titration Calorimetry, DNA, Thermodynamics, Gold nanoparticles

Citation

Lang, B. (2010), Hybridization Thermodynamics of Bound DNA, Journal of the American Chemical Society, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=901435 (Accessed June 24, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created August 8, 2010, Updated March 24, 2017