High-Throughput Methods to Assay Cell Shape and Response on Topographically Patterned Surfaces
S L. Kennedy, N Washburn, A Sehgal, Alamgir Karim, Eric J. Amis
Numerous groups have demonstrated that cell alignment, cell spreading and cell proliferation can be controlled by various topographical features. There are still major outstanding questions to be addressed in the area of topographical affects on cell adhesion, growth and proliferation. Several experimental variables can be used to define topography and it would be tedious to explore each variable on individually prepared substrates. It is advantageous to apply a high-throughput approach to study the effect of cellular interactions with surfaces. Toward this goal, we have taken the first step in demonstrating that a high throughput approach can be used to study the interaction of cells on a topographical surface. Grooved DL-polylactide coated surfaces with a step gradient in pattern dimensions were prepared using conventional photolithographic methods. The response of MC3T3-E1 osteoblasts cell on these surfaces were measured and compared with literature results.
cell, patterning, polymer lithography, topography
, Washburn, N.
, Sehgal, A.
, Karim, A.
and Amis, E.
High-Throughput Methods to Assay Cell Shape and Response on Topographically Patterned Surfaces, Biomaterials
(Accessed May 29, 2023)