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Failure mechanisms in DNA self-assembly: Barriers to single fold yield



Jacob Majikes, Paul Patrone, Anthony J. Kearsley, Michael P. Zwolak, James Liddle


Understanding the folding process of DNA origami is a critical steppingstone to the broader implementation of nucleic acid nanofabrication technology but is notably non-trivial. Origami are formed by several hundred cooperative hybridization events – folds – between spatially separate domains of a scaffold, derived from a viral genome, and oligomeric staples, each of which is difficult to detect independently. Here, we present a real-time probe of the unit operation of origami assembly, a single fold across the scaffold as a function of hybridization domain separation – fold distance – and staple:scaffold ratio. This novel approach to the folding problem elucidates a previously unobserved blocked state that acts as a limit on yield for single folds, and which may manifest as a barrier in whole origami assembly.
ACS Nano


DNA origami, nanofabrication


Majikes, J. , Patrone, P. , , A. , Zwolak, M. and Liddle, J. (2021), Failure mechanisms in DNA self-assembly: Barriers to single fold yield, ACS Nano, [online], , (Accessed April 23, 2024)
Created February 23, 2021, Updated April 6, 2021