Failure mechanisms in DNA self-assembly: Barriers to single fold yield
Jacob Majikes, Paul N. Patrone, Anthony Kearsley, Michael P. Zwolak, James Liddle
Understanding the folding process of DNA origami is a critical steppingstone to the broader implementation of nucleic acid nanofabrication technology but is notably non-trivial. Origami are formed by several hundred cooperative hybridization events – folds – between spatially separate domains of a scaffold, derived from a viral genome, and oligomeric staples, each of which is difficult to detect independently. Here, we present a real-time probe of the unit operation of origami assembly, a single fold across the scaffold as a function of hybridization domain separation – fold distance – and staple:scaffold ratio. This novel approach to the folding problem elucidates a previously unobserved blocked state that acts as a limit on yield for single folds, and which may manifest as a barrier in whole origami assembly.
, Patrone, P.
, Kearsley, A.
, Zwolak, M.
and Liddle, J.
Failure mechanisms in DNA self-assembly: Barriers to single fold yield, ACS Nano, [online], https://doi.org/10.1021/acsnano.0c10114 , https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=930700
(Accessed April 18, 2021)