Skip to main content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodyanmic focusing device

Published

Author(s)

Justin M. Zook, Wyatt N. Vreeland

Abstract

Microfluidic hydrodynamic focusing of an alcohol/lipid mixture into a narrow fluid stream by two oblique buffer streams provides a controlled and reproducible method of forming phospholipid bilayer vesicles (i.e., liposomes) with relatively monodisperse and specific size ranges. Previous work has established that liposome size can be controlled by changing the relative and absolute flow rates of the fluids. A kinetic (non-equilibrium) theoretical description of the detergent dilution liposome formation method was developed previously, in which planar bilayer discs aggregate until they become sufficiently large to close into spherical liposomes. In this work, we use the kinetic theory to predict that the temperature should change the liposome size primarily as a result of its effect on the membrane bending elasticity modulus. In agreement with theory, our experiments show larger liposomes forming at or below the transition temperature, and a much smaller dependence of size on temperature well above the transition temperature, where the elasticity modulus is relatively constant.
Citation
Soft Matter
Volume
6
Issue
6
Created March 21, 2010, Updated February 19, 2017