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Effects of nanoparticle size and charge on interactions with self-assembled collagen



Dongbo Wang, Jing (. Ye, Steven Hudson, Keana Scott, Sheng Lin-Gibson


Recent insights into bone formation have suggested that the critical first step to the biomineralization process is the integration of small (nanometer dimension) mineral clusters into collagen fibers. Such behavior is of intrinsic interest for the areas of biomineralization and directed self assembly of hierarchical structures but also to nanotoxicology, because collagen is a major component of structural tissues in the human body and accounts for more than 25 % of the whole body protein content. This study uses Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) with complementary imaging (AFM and SEM) to explore the affinity of nanoparticles of different sizes and surface charge polarities with self-assembled collagen fibers. Importantly, we find that both positively and negatively charged nanoparticles exhibited binding affinity for collagen and only the smallest particle examined (2 nm) mechanically integrated with collagen fibers.
Journal of Colloid and Interface Science


Adsorption, QCM-D, toxicity, biomineralization


Wang, D. , Ye, J. , Hudson, S. , Scott, K. and Lin-Gibson, S. (2014), Effects of nanoparticle size and charge on interactions with self-assembled collagen, Journal of Colloid and Interface Science, [online],, (Accessed June 23, 2024)


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Created February 28, 2014, Updated October 12, 2021