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Effect of Collagen in Calcium Phosphate Cement on Mechanical and Cellular Properties



Jennifer L. Moreau, Michael Weir, Hockin D. Xu


Calcium phosphate cement (CPC) can be molded to conform to complex bone cavities and set in situ to form bioresorbable hydroxyapatite. The aim of this study was to develop a biomimetic nano apatite-collagen fiber composite, and to investigate the effect of collagen on mechanical and cellular properties. A type I bovine collagen powder (Sigma Aldrich) was added to CPC to develop a bone-mimicking, nano apatite-collagen composite. Work-of-fracture (toughness), measured in three-point flexure (mean sd; n = 6), was increased from (9.4 3.1) J/m2 for CPC with 0 % collagen, to (430 103) J/m2 for CPC containing 4.05 % collagen (p 0.1); at P/L = 3.5, collagen incorporation slightly decreased the flexural strength.SEM examination revealed coverage of collagen fibers by nano apatite crystals with intimate contact between the two. Increasing the collagen volume fraction in CPC significantly increased the number of MC3T3-E1 osteoblast cell attachment (p


bone tissue engineering, cell attachment, collagen fibers, live cell density, phosphate cement, strength, work-of-fracture


Moreau, J. , Weir, M. and Xu, H. (2008), Effect of Collagen in Calcium Phosphate Cement on Mechanical and Cellular Properties, Biomaterials, [online], (Accessed May 18, 2024)


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Created December 23, 2008, Updated February 19, 2017