Polyethyleneimine (PEI) functionalized AuNPs (Au-PEIs) are have potent as positively charged gold nanoparticles (AuNPs) for nano-medicinal applications, due to their cationic surfaces that promote cellular uptake and gene transfection. 1-6 1-6 1-61-6 Au-PEIs can be prepared by one of two methods: (1) ligand modification (exchange or co-attachment) of AuNPs and PEI, and (2) reduction of AuIII ions in the presence of PEI. Herein, the ligand exchanged Au-PEIs (Au- PEI[LE]s) and the materials by reduction (Au-PEI[Red]s) were prepared from citrate-stabilized AuNPs (10 nm, nominal) and HAuCl4, respectively. We demonstrate differentiated product formation via each synthetic route and their discriminated physico-chemical properties by systematic examination. The physico-chemical properties and the conjugation mechanisms of Au- PEI[LE] and Au-PEI[Red] were characterized by orthogonal analyses system. Furthermore, the colloidal stabilities of all produced Au-PEIs were investigated under various physiologically relevant conditions and shown that the consequential fates of Au-PEIs were dependent on born pathways significantly. We found that some of Au-PEIs showed outstanding stability in certain circumstances which is very critical to be a drug carrier in nanomedicine.
The TechConnect Briefs
June 17-19, 2019
TechConnect World Innovation Conference 2019
"gold nanoparticles", "polyethyleneimine", "PEI", "gold PEI conjugates", "positively charged", "synthesis", "characterization", "stability"