Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Direct Spectroelectrochemical Titration of Glutathione



Vytautas Reipa


Spectroelectrochemistry measurements are used to determine the formal potential E of glutathione. Nernst titration was performed by monitoring disulfide absorption at 258 nm in a thin layer cell. Use of nanocrystalline highly doped tin oxide electrodes allowed the repeated direct glutathione reduction and oxidation. At pH = 7.35, T = 298K, GSSG range from 1 to 5 mM, and 1=0.1 mol/kg, the measured value of E = -22 + 0.02 V (NHE).
Chemical Communications


formal poetntial redox reaction, glutathione, NADP, UV-VIS spectroscopy


Reipa, V. (2004), Direct Spectroelectrochemical Titration of Glutathione, Chemical Communications (Accessed April 23, 2024)
Created December 1, 2004, Updated February 17, 2017