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Direct Spectroelectrochemical Titration of Glutathione
Published
Author(s)
Vytautas Reipa
Abstract
Spectroelectrochemistry measurements are used to determine the formal potential E of glutathione. Nernst titration was performed by monitoring disulfide absorption at 258 nm in a thin layer cell. Use of nanocrystalline highly doped tin oxide electrodes allowed the repeated direct glutathione reduction and oxidation. At pH = 7.35, T = 298K, GSSG range from 1 to 5 mM, and 1=0.1 mol/kg, the measured value of E = -22 + 0.02 V (NHE).