Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Differences in PDMS Modification Affect Laminin Deposition and Smooth Muscle Cell Response

Published

Author(s)

Joy P. Dunkers, Hae-Jeong Lee, Marvi Matos, Lisa Pakstis, Juan M. Taboas, Steven D. Hudson, Marcus T. Cicerone

Abstract

When culturing cells on flexible surfaces, it is important to consider extracellular matrix treatments that will remain on the surface under mechanical strain. Here we investigate differences in laminin surfaces that were deposited on oxidized PDMS (plasma only) versus oxidized PDMS treated with aminopropyltrimethoxysilane (silane-linked). We use specular X-ray reflectivity (SXR), transmission electron microscopy (TEM), and immunofluorescence to probe the quantity and uniformity of the laminin on the treated PDMS surfaces. We compare vascular smooth muscle cell (SMC) proliferation on laminin under equibiaxial strain between plasma only and silane-linked treatments.
Proceedings Title
American Chemical Society Division of Polymeric Materials: Science and Engineering Meeting| |Proceedings of the ACS Division of Polymeric Materials: Science & Engineering |ACS
Volume
101
Conference Dates
August 16-20, 2009
Conference Location
Washingont, DC

Keywords

specular X-ray reflectivity, transmission electron microscopy, laminin, PDMS, smooth muscle cell

Citation

Dunkers, J. , Lee, H. , Matos, M. , Pakstis, L. , Taboas, J. , Hudson, S. and Cicerone, M. (2009), Differences in PDMS Modification Affect Laminin Deposition and Smooth Muscle Cell Response, American Chemical Society Division of Polymeric Materials: Science and Engineering Meeting| |Proceedings of the ACS Division of Polymeric Materials: Science & Engineering |ACS, Washingont, DC, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=901800 (Accessed April 15, 2024)
Created August 16, 2009, Updated February 19, 2017