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Developmental Validation of the VersaPlex® 27PY System

Published

Author(s)

Kristy Lenz, Stephen McDaniel, Dawn Rabbach, Becky Steffen, Douglas Storts, Robert McLaren

Abstract

The VersaPlex™ 27PY System is a short tandem repeat (STR) multiplex from Promega that co-amplifies 27 loci. This six-dye multiplex was designed for casework samples and includes loci that meet database requirements for the US (CODIS) and Europe (ESS). The 23 autosomal loci amplified by the VersaPlex™ 27PY System have high powers of discrimination and include the D6S1043 locus, which is especially informative for Asian populations. The multiplex also includes two rapidly mutating Y-STRs that can provide useful genetic information for forensic samples. In this article we describe the developmental validation experiments using the VersaPlex™ 27PY System to meet SWGDAM requirements. We document how reaction variables including PCR inhibitors, reaction volume, cycle number, annealing temperature and magnesium concentration can affect multiplex performance. We also evaluate the sensitivity, specificity, precision and reproducibility of the VersaPlex™ 27PY System. The results of the experiments demonstrate the capabilities of the VersaPlex™ 27PY System as a tool for forensic laboratories.
Citation
Promega Corporation

Keywords

VersaPlex™, Short tandem repeat (STR), Validation, Forensic science, Developmental validation, CODIS, ESS, Casework, Database, 6 dye, Y-STR, SWGDAM

Citation

Lenz, K. , McDaniel, S. , Rabbach, D. , Steffen, B. , Storts, D. and McLaren, R. (2023), Developmental Validation of the VersaPlex® 27PY System, Promega Corporation, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=936919, https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpromega.widen.net%2Fs%2Fzxgmlwgj77%2Fversaplex-27py-system-technical-note-an375&data=05%7C01%7Cbecky.steffen%40nist.gov%7Cb6c7f94ded394349a27308dbe49967ee%7C2ab5d82fd8fa4797a93e054655c61dec%7C1%7C0%7C638355119789257713%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=cQTYOKPXtIvinOGE3HPL7J5n%2F4hNvlUmHvNZjk9wUSA%3D&reserved=0 (Accessed March 5, 2024)
Created November 21, 2023, Updated November 29, 2023