Development of Kudzu (Pueraria montana var. lobata) Reference Materials for the Determination of Isoflavones and Toxic Elements

Published

February 21, 2022

Author(s)

J. Andreas Lippert, Kate Rimmer, Melissa M. Phillips, Michael Nelson, Chuck Barber, Laura J. Wood, Marie Ale, Kaitlyn Chieh, Stephen Long, Colleen E. Bryan Sallee, Shannon Whitehead, Katherine E. Sharpless, James H. Yen, Danica Raynaud, Joesph Betz, Adam Kuszak, Stephen Wise

Abstract

As part of a collaboration with the Office of Dietary Supplements at the National Institutes of Health (NIH-ODS), the National Institute of Standards and Technology (NIST) recently issued a suite of new botanical matrix Reference Materials (RMs) and Standard Reference Materials® (SRMs) for the determination of isoflavones and toxic elements in kudzu dietary supplement ingredients. RM 8650 Pueraria montana var. lobata (Kudzu) Rhizome, SRM 3268 Pueraria montana var. lobata (Kudzu) Extract, and RM 8652 Kudzu-Containing Solid Oral Dosage Form were issued with values assigned for three isoflavones (puerarin, daidzin, and daidzein) and toxic elements (arsenic, cadmium, and lead) and selenium. Mass fraction values assigned for the isoflavones were based on measurements using liquid chromatography with UV absorbance or mass spectrometry detection. The element content was assigned for two of the materials (rhizome and extract) based on results from inductively coupled plasma mass spectrometry (ICP-MS) and results from an interlaboratory comparison exercise. The kudzu RM/SRM suite complements previously issued soy-related SRMs with values assigned for isoflavones, phytochemicals that have been studied for their potential health benefits, and expands the analytical resource by providing values for puerarin, which is an isoflavone not found in soy.

Citation

Journal of AOAC International

Volume

105

Issue

4

Pub Type

Journals

Download Paper

https://doi.org/10.1093/jaoacint/qsac023

Local Download

Keywords

reference material (RM), kudzu, botanical dietary supplement ingredient, liquid chromatography – mass spectrometry (LC-MS), isoflavones, toxic metals

Citation

Lippert, J. , Rimmer, K. , Phillips, M. , Nelson, M. , Barber, C. , Wood, L. , Ale, M. , Chieh, K. , Long, S. , Bryan Sallee, C. , Whitehead, S. , Sharpless, K. , Yen, J. , Raynaud, D. , Betz, J. , Kuszak, A. and Wise, S. (2022), Development of Kudzu (Pueraria montana var. lobata) Reference Materials for the Determination of Isoflavones and Toxic Elements, Journal of AOAC International, [online], https://doi.org/10.1093/jaoacint/qsac023, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=932792 (Accessed October 8, 2025)