Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Development of Kudzu (Pueraria montana var. lobata) Reference Materials for the Determination of Isoflavones and Toxic Elements



J. Andreas Lippert, Kate Rimmer, Melissa M. Phillips, Michael Nelson, Chuck Barber, Laura J. Wood, Marie Ale, Kaitlyn Chieh, Stephen Long, Colleen E. Bryan Sallee, Shannon Whitehead, Katherine E. Sharpless, James H. Yen, Danica Raynaud, Joesph Betz, Adam Kuszak, Stephen Wise


As part of a collaboration with the Office of Dietary Supplements at the National Institutes of Health (NIH-ODS), the National Institute of Standards and Technology (NIST) recently issued a suite of new botanical matrix Reference Materials (RMs) and Standard Reference Materials® (SRMs) for the determination of isoflavones and toxic elements in kudzu dietary supplement ingredients. RM 8650 Pueraria montana var. lobata (Kudzu) Rhizome, SRM 3268 Pueraria montana var. lobata (Kudzu) Extract, and RM 8652 Kudzu-Containing Solid Oral Dosage Form were issued with values assigned for three isoflavones (puerarin, daidzin, and daidzein) and toxic elements (arsenic, cadmium, and lead) and selenium. Mass fraction values assigned for the isoflavones were based on measurements using liquid chromatography with UV absorbance or mass spectrometry detection. The element content was assigned for two of the materials (rhizome and extract) based on results from inductively coupled plasma mass spectrometry (ICP-MS) and results from an interlaboratory comparison exercise. The kudzu RM/SRM suite complements previously issued soy-related SRMs with values assigned for isoflavones, phytochemicals that have been studied for their potential health benefits, and expands the analytical resource by providing values for puerarin, which is an isoflavone not found in soy.
Journal of AOAC International


reference material (RM), kudzu, botanical dietary supplement ingredient, liquid chromatography – mass spectrometry (LC-MS), isoflavones, toxic metals


Lippert, J. , Rimmer, K. , Phillips, M. , Nelson, M. , Barber, C. , Wood, L. , Ale, M. , Chieh, K. , Long, S. , Bryan Sallee, C. , Whitehead, S. , Sharpless, K. , Yen, J. , Raynaud, D. , Betz, J. , Kuszak, A. and Wise, S. (2022), Development of Kudzu (Pueraria montana var. lobata) Reference Materials for the Determination of Isoflavones and Toxic Elements, Journal of AOAC International, [online],, (Accessed July 17, 2024)


If you have any questions about this publication or are having problems accessing it, please contact

Created February 21, 2022, Updated December 7, 2022