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Determination of Isoflavone Content in SRM 3238 Using Liquid Chromatography-Particle Beam/Electron Ionization Mass Spectrometry



Lynn Zhang, Carolyn Burdette, Melissa M. Phillips, Kate Rimmer, R. K. Marcus


The characterization of active components in botanical materials is a challenging task, especially as increased consumption of botanicals and dietary supplements demands a greater understanding of the associated health benefits and risks. In order to successfully acquire and compare clinical results and correlate health trends, accurate, precise, and validated methods of analysis must be developed. Presented here is the development of a quantitative method of analysis of soy isoflavones (daidzin, glycitin, genistin, daidzein, and genistein) using liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS). A 4 h rotary mixing extraction procedure, with 74.5:20.0:5.5 (v:v:v) methanol:water:DMSO as the extraction solvent, was employed prior to a reversed-phase separation on a commercial C18 column with a gradient of water and methanol (both containing 0.1 % acetic acid). An internal standard (IS) approach for quantitation with 7-hydroxy-4-chromone as the IS compound was employed, with response factors for each target isoflavone calculated. This method was compared directly with the results obtained by an NIST method, using SRM 3238 Soy-Containing Solid Oral Dosage Form as the test sample matrix, for method validation. The LC-PB/EIMS approach provides accurate and precise measurements for the determination of the target isoflavones as well as providing simultaneous structural information.
Journal of Aoac International


Isoflavones, PB-MS, particle beam, mass spectrometry, SRM 3238


Zhang, L. , Burdette, C. , Phillips, M. , Rimmer, K. and Marcus, R. (2015), Determination of Isoflavone Content in SRM 3238 Using Liquid Chromatography-Particle Beam/Electron Ionization Mass Spectrometry, Journal of Aoac International, [online], (Accessed April 24, 2024)
Created October 31, 2015, Updated January 4, 2022