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Cryosalts: Suppression of Ice Formation in Macromolecular Crystallography

Published

Author(s)

Kenneth A. Rubinson, Jane E. Ladner, M Tordova, G L. Gilliland

Abstract

Quality data collection for macromolecular cryocrystallography requires suppressing the formation of crystalline or microcrystalline ice that may result from flash-freezing crystals. Described here is the use of lithium formate, lithium chloride, and other highly soluble salts for forming ice-ring -free aqueous glasses upon cooling from ambient temperature to 100 K. These cryosalts are a new class of cryoprotectants that are shown to be effective with a variety of commonly used crystallization solutions and with proteins crystallized under different conditions. The influence of cryosalts on crystal mosaicity and diffraction resolution is comparable or superior to traditional organic cryoprotectants.
Citation
ACTA Crystallographica Section D-Biological Crystallography
Volume
56
Issue
Part 8

Keywords

aqueous glasses, cryocrystallography, cryoprotectant, cryosalt, lithium chloride, lithium formate, sodium chloride, sodum formate

Citation

Rubinson, K. , Ladner, J. , Tordova, M. and Gilliland, G. (2000), Cryosalts: Suppression of Ice Formation in Macromolecular Crystallography, ACTA Crystallographica Section D-Biological Crystallography (Accessed April 18, 2024)
Created July 31, 2000, Updated October 12, 2021