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Control experiments to avoid artifacts and misinterpretations in nanoecotoxicology testing



Elijah J. Petersen


This special publication is one in a series stemming from the National Nanotechnology Initiative (NNI) Nano-EHS Research Strategy which identified Nanomaterial Measurement Infrastructure as one of the essential areas of research needed in order to develop an effective risk assessment and management plan regarding various aspects of nanotechnology in consumer products as it pertains to human health, exposure and the environment. The National Institute of Standards and Technology (NIST) was identified as a lead agency in the development of measurement strategies for the robust development to assess the potential effects of engineered nanomaterials and their fate in the environment. One important factor in these measurements is avoidance of artifacts and misinterpretations in nanoecotoxicology experiments. The current guidance document presents potential control experiments to include during nanoecotoxicology testing to minimize artifacts and misinterpretations and to improve data reliability. Updates to this protocol may be released in the future. Visit measurement-protocols.cfm to check for revisions of this protocol, or new protocols in the series. We also encourage users to report citations to published work in which this protocol has been applied.
Special Publication (NIST SP) - 1200-11
Report Number


Petersen, E. (2015), Control experiments to avoid artifacts and misinterpretations in nanoecotoxicology testing, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], (Accessed June 17, 2024)


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Created May 22, 2015, Updated November 10, 2018